用智能手机也可以检测新冠病毒了手机有新冠病毒吗(12) _小知识

pandemic 流行病 ; 大流行病 ; 大流行的 ; 普遍的，全世界的
that is 即 ; 用于纠正之前说过的内容
circulating 环流，循环 ; 传播 ; 流传 ; 散布 ; 传送 ; 传递 ; 传阅 ; 运行的 ; circulate的现在分词
low-tech 不涉及最现代技术的
low cost 低成本；廉价
scalable 可攀登的；可去鳞的；可称量的
head-to-head 正面交锋的 ; 面对面的 ; 肉搏战 ; 白刃战
diagnostic test 诊断测验
false positives 假阳性 ; false positive的复数
in addition to 另外，加之，除…之外
room temperature 室温 ; 常温
may be 多半
The smartphone-based LAMP assay detection system consists of a hot plate, cardboard box, and LED lights. The system is inexpensive to set up and portable; it can be fabricated for less than $100 (in addition to the smartphone cost, which is approximately $200 used or approximately $400 new). The smartphone-based LAMP assay thus offers the potential to leverage a readily accessible technology to inexpensively deliver state-of-the-art nucleic acid diagnostics for quantitative pathogen detection at the POC. As reported in this study and by others, SARS-CoV-2 can be detected in saliva samples.27,45,46 Saliva testing has numerous advantages relative to nasopharyngeal swabs, including cost and ease of use (self-collection vs trained personnel and PPE for sample collection). Furthermore, saliva sampling circumvents the need for specialized swabs and reagents, test components that are vulnerable to supply-chain disruptions and resource limitations.

基于智能手机的 LAMP 检测系统由热板、纸板箱和 LED 灯组成。该系统的安装成本低廉且便于携带；它的制造成本不到 100 美元（除了智能手机成本，大约 200 美元使用或大约 400 美元新）。因此，基于智能手机的 LAMP 检测提供了利用一种易于获得的技术以低成本提供最先进的核酸诊断技术的潜力，用于在 POC 进行定量病原体检测。正如本研究和其他人所报道的，可以在唾液样本中检测到 SARS-CoV-2 。27 , 45 , 46与鼻咽拭子相比，唾液检测具有许多优势，包括成本和易用性（自我采集与受过培训的人员和用于样本采集的 PPE）。此外，唾液采样无需专门的拭子和试剂、易受供应链中断和资源限制的测试组件。
detection system 检测系统；探测系统
hot plate 电热板；轻便电炉；餐厅出售的热食
less than 少于 ; 毫不 ; 完全不 ; 一点都不
in addition to 另外，加之，除…之外
nucleic acid 核酸
relative to 关于…的，和…比较起来

There are opportunities to adapt our protocol from a lab test to a field test and to further reduce the cost of smartphone-based LAMP diagnostics. First, because approximately 90% of the cost per test comes from using commercially available enzymes, bulk enzyme purification or purchase could further lower the cost substantially. Second, field-test applicability could conceivably be achieved with the use of lyophilized reagents, which would be well-suited for areas that lack refrigeration.47,48 The use of lyophilized reagents can further streamline the LAMP assay by enabling the addition of specimen directly to a preassembled master mix, thereby minimizing sample handling, preparation time and the potential for user error, while enhancing user biosafety. Additionally, the system provides a platform for inexpensive home-based testing.

有机会将我们的协议从实验室测试调整为现场测试，并进一步降低基于智能手机的 LAMP 诊断成本。首先，由于每次测试大约 90% 的成本来自使用市售酶，因此批量酶纯化或购买可以进一步显着降低成本。其次，可以想象使用冻干试剂可以实现现场测试的适用性，这将非常适合缺乏冷藏的地区。47 , 48冻干试剂的使用可以通过将样品直接添加到预组装的预混液中来进一步简化 LAMP 检测，从而最大限度地减少样品处理、制备时间和用户错误的可能性，同时提高用户的生物安全性。此外，该系统还为廉价的家庭测试提供了平台。
There are 有 ; 可数名词的复数形式 ; 许多重要事情要谈
field test 现场试验
commercially available 市场上可买到的
field-test 对做现场试验
conceivably 可想而知 ; 想得到地
lyophilized 使冻干 ; lyophilize的过去分词和过去式
preparation time 预备时间

The capacity to rapidly and accurately test large populations, including individuals in developing nations struggling with inadequate vaccine supplies and testing access amidst a landscape of new, and more highly transmissible variants, is critically important. Accordingly, the smartphone-based LAMP assay detected 5 of 5 major SARS-CoV-2 variants including Alpha and Delta. Through primer changes, the LAMP assay could be further adjusted as necessary if new, as yet unseen, variants evolve. The Omicron variant, which emerged during the manuscript review process and possesses approximately 50 variations with many clustered in the gene encoding the spike protein,49 would not be expected to escape diagnostic detection of the LAMP primers described in this study. The broad applicability of a smartphone-based assay has been previously demonstrated with excellent performance for urinary bacterial diagnostics and for the detection of multiple microbial pathogens in body fluids (blood, urine, feces).50 The LAMP assay now potentially offers underserved populations with an additional testing tool for the next stage in the pandemic. Moreover, the lifting of COVID pandemic restrictions is expected to markedly increase influenza cases during the upcoming flu season and has prompted CDC recommendations for SARS-CoV-2 and influenza combination diagnostics when both pathogens are circulating.17,51 Finally, integration of the smartphone-based LAMP assay with telemedicine has the potential to deliver advanced health care to vulnerable populations, while markedly broadening the scope of personalized medicine.52,53 Taken together, the LAMP assay integrates state-of-the-art diagnostic techniques with the connectivity and computational power of the smartphone, offering the potential to provide fair and equal access to precision diagnostic medicine.

用智能手机也可以检测新冠病毒了 手机有新冠病毒吗(12)

用智能手机也可以检测新冠病毒了手机有新冠病毒吗(12)